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Srinivasa Murthy, K.
- Assessment of Genetic Variation in Cotesia flavipes Cameron (Hymenoptera: Braconidae) Populations as Revealed by Mitochondrial Cytochrome Oxidase Gene Sequences
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Authors
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1 National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Bangalore 560024, Karnataka, IN
1 National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Bangalore 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 23, No 3 (2009), Pagination: 249-253Abstract
The cytochrome oxidase gene (COI) was employed to assess the genetic variation in different populations of Cotesia flavipes. Partial sequences of the COI gene for the populations from Bangalore, Hyderabad, New Delhi and Shimla were analyzed to assess the homology and the usefulness of this genetic region for phylogenetic studies. PCR using COI-F and COI-R primers amplified a product of approximately 550 bp which was similar for all the four populations. Populations were by and large similar in the COI gene sequenced and there was no variation with our sequences and those of sequences worldwide. The multiple alignments were performed for four populations which revealed similarity of the partial COI gene sequences. Comparative analysis of partial sequences of COI gene produced a phylogenetic tree. Phylogenetic analysis revealed that all our populations were in a single clade with high boot strap value, suggesting highest similarity.Keywords
Cotesia flavipes, Cytochrome Oxidase, Genetic Variation.Full Text
References
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- Rattan, R. S., Reineke, A., Ashok, H., Gupta, P. R. andZebitz, C. P. W. 2006. Molecular phylogeny ofCotesia species (Hymenoptera: Braconidae)inferred from a 16s gene. Current Science, 91:1460–1461.
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- Influence of Parasitoid-Host Density on the Behaviour Ecology of Goniozus nephantidis (Muesebeck) (Hymenoptera: Bethylidae), a Parasitoid of Opisina arenosella Walker
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Authors
Affiliations
1 National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Hebbal, Bangalore 560024, Karnataka, IN
1 National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Hebbal, Bangalore 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 23, No 3 (2009), Pagination: 255-264Abstract
Since Goniozus nephantidis (Muesebeck) is found to have strong parental care for its progeny, the influence of different densities of G. nephantidis and its host, Corcyra cephalonica (Stainton) on the behaviour ecology of the parasitoid was studied. Interaction between different densities of the host and parasitoid revealed that a ratio of 1:1 was significantly superior to all other ratios resulting in maximum parasitism (9.0 larvae / female), fecundity (93.2/female) and number of progenies (75.2/female). Exposing more than one C. cephalonica larva did not significantly increase the parasitizing efficiency, fecundity and progeny produced. Conversely, exposing a single C. cephalonica larva to several female parasitoids adversely affected the biological attributes of the parasitoid. Increasing the densities of either host insects or parasitoids had an inverse relationship with oviposition behaviour, parasitism efficiency and progeny production of the parasitoid establishing the important and significant role played by host-parasitoid density.Keywords
Corcyra cephalonica, Density, Goniozus nephantidis, Opisina arenosella, Parasitizing Behaviour, Parental Care.Full Text
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- Biochemical and Molecular Diversity Analysis of Culturable Bacteria in Cotesia plutellae (Kurdjumov) (Hymenoptera: Braconidae), a Parasitoid of Diamondback Moth, Plutella xylostella (Linnaeus)
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Authors
Affiliations
1 Division of Molecular Entomology, National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore 560 024, IN
1 Division of Molecular Entomology, National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore 560 024, IN
Source
Journal of Biological Control, Vol 27, No 4 (2013), Pagination: 260–267Abstract
Geographical populations of Cotesia plutellae, a predominant endolarval parasitoid of the diamondback moth, Plutella xylostella (Linnaeus) were screened and analyzed for bacterial diversity. The culturable bacterial species were isolated and identified by sequence analysis of 16S rRNA gene. Eleven bacterial isolates were identified viz., Pseudomonas sp., Enterobacter cancerogenus, Bacillus spp., Pseudomonas putida, Pantoea agglomerans, Bacillus thuringiensis, Pantoea sp. and Bacillus cereus. The molecular characterization and phylogenetic analysis placed these phylotypes into two major classes i.e. Bacilli and Gamma proteobacteria. The evolutionary distance matrix (Pairwise distance) showed similarity between the sequences. The bacterial diversity observed was low in the different geographic populations. The nucleotide sequences were blasted and submitted to GenBank.Keywords
Bacteria, Cotesia plutellae, 16S rRNA, Symbiont.Full Text
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- Molecular Characterisation of some Indian Anthocorid Predators
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Authors
Affiliations
1 ICAR-National Bureau of Agricultural Insect Resources, P B.No. 2491, H.A. Farm Post, Bellary road, Bengaluru – 560024, IN
1 ICAR-National Bureau of Agricultural Insect Resources, P B.No. 2491, H.A. Farm Post, Bellary road, Bengaluru – 560024, IN
Source
Journal of Biological Control, Vol 29, No 1 (2015), Pagination: 8-13Abstract
Anthocorid predators widely used as biocontrol agents were characterised for genetic diversity and phylogeny. Cardiastethus exiguus Poppius, Xylocoris flavipes Reuter and Blaptostethus pallescens Poppius were characterised using Cytochrome oxidase I (COI) marker to elucidate the diversity and phylogeny. The sequencing of the COI region at 650bp was compared with sequence of Orius majusculus (Reuter), available at NCBI by BLASTn. The cluster analysis based on dendogram revealed B. pallescens and O. majusculus to be more closely related, while X. flavipes and C. exiguus were distinct. The usefulness of characterisation and phylogenetic relationship among the anthocorid populations for their effective utilisation in pest management is discussed.Keywords
Anthocorids, COI, PCR, Phylogeny, Predators.Full Text
- Development of Crocidolomia binotalis Zeller (Lepidoptera: Pyralidae) on a Semi Synthetic Diet
Abstract Views :226 |
PDF Views:116
Authors
Affiliations
1 Project Directorate of Biological Control (ICAR), Post Bag No.2491, H. A. Farm Post, Bellary Road, Bangalore, 560 024, Karnataka, IN
1 Project Directorate of Biological Control (ICAR), Post Bag No.2491, H. A. Farm Post, Bellary Road, Bangalore, 560 024, Karnataka, IN
Source
Journal of Biological Control, Vol 19, No 2 (2005), Pagination: 179-182Abstract
The mean developmental period of Crocidolomia binotalis Zeller was 32.5 days with a percentage larval survival of 65.1per cent and adult emergence of 56.6 per cent on a semi-synthetic diet, while these were 26.6 days, 79.1 and 83.2%, respectively when reared on cabbage leaves. The factors for the variations in the biological attributes when reared on the diets and the feasibility of utilizing semi-synthetic diet reared host for rearing the parasitoids are discussed.Keywords
Biological Parameters, Cabbage Leaf Webber, Crocidolomia binotalis, Semi-Synthetic Diet, Rearing.Full Text
- A Novel Method of Field Release of Goniozus nephantidis (Muesebeck), an Important Primary Parasitoid of Opisina arenosella Walker on Coconut
Abstract Views :275 |
PDF Views:121
Authors
Affiliations
1 Project Directorate of Biological Control (ICAR) Post Bag No. 2491, Bellary Road, Hebbal, Bangalore 560 024, Karnataka, IN
1 Project Directorate of Biological Control (ICAR) Post Bag No. 2491, Bellary Road, Hebbal, Bangalore 560 024, Karnataka, IN
Source
Journal of Biological Control, Vol 17, No 1 (2003), Pagination: 79-80Abstract
Goniozus nephantidis (Muesebeck) is an important primary parasitoid of Opisina arenosella Walker in coconut eco-system. The method of release of G. nephantidis was standardized for the first time. It was found that 90-100 per cent of the parasitoids released at the trunk (1.2 m from the ground level) reached the crown of the palm irrespective of the height of the tree. The mean time taken by each batch of the parasitoids to reach the crown varied from 13 to 22 minutes. It was also found that the parasitoids preferred to crawl on the tree rather than flying. Hence it is suggested that G. nephantidis adults may be released at the trunk (1.2 m height from the ground level) of the coconut palm for the management of O. arenosella instead of releasing at the crown region of the palm or arbitrarily on unit area basis.Keywords
Goniozus nephantidis Method of Release, Opisina arenosella.Full Text
- New Record of Carinostigmus Tsuneki (Hymenoptera: Crabronidae: Pemphredoninae) Species in India and Identity of its Species using DNA Barcoding
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Authors
Affiliations
1 Division of Genomic Resources, ICAR - National Bureau of Agricultural Insect Resources,Bangalore - 560024, Karnataka, IN
1 Division of Genomic Resources, ICAR - National Bureau of Agricultural Insect Resources,Bangalore - 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 33, No 1 (2019), Pagination: 70-75Abstract
Specimens of the aphid hunting wasp Carinostigmus Tsuneki (Hymenoptera: Crabronidae: Pemphredoninae) were collected from South India. Morphological identification revealed three species, and one of them, C. griphus Krombein, is new for India. Identification of the species is supported through COI partial gene-DNA Barcoding.Keywords
Carinostigmus, DNA Barcoding, Molecular Phylogeny, Sphecidae.Full Text
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